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KMID : 0364819860240030295
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Korean Journal of Microbiology
1986 Volume.24 No. 3 p.295 ~ p.301
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Isolation of β-1, 3-Glucanase Producing Strain and Cultural Conditions of Its Enzyme Production
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Abstract
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1
The bacteria, which were capable of producing p-1, 3-glucanase inducibly by utilizing cell wall of Aspergillus fumigatus as a sole carbon source, were isolated from soil in the campus of Kyungpook National University. Among them, the strain which produced the enzyme excellently was selected and identified to be Pseudomonas stutzeri KF 13 by morphological, cultural and physiological examination.The optimal conditions for the enzyme production from Pseudomonas stutzeri KF 13 were investigated. The enzyme production was reached maximum state when the broth cultured for 72hr at 30C. And the enzyme showed the highest activity in the medium containing 3.5% cell wall as an inducer, 15% yeast autolysate as a nitrogen source and 0.05% MnSO, at pH 7.5.
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